Web Application in Virtual Directory

I'm running Kentico CMS 5.0 at the root of the web site. I've created a sub directory and made it a Virtual Directory to run another web application which is not a Kentico CMS web application. I'm receiving the following error when running a page from the application in the virtual directory:

Request is not available in this context
Description: An unhandled exception occurred during the execution of the current web request. Please review the stack trace for more information about the error and where it originated in the code.

Code:

Exception Details: System.Web.HttpException: Request is not available in this context

Source Error: 

An unhandled exception was generated during the execution of the current web request. Information regarding the origin and location of the exception can be identified using the exception stack trace below.  

Stack Trace: 


[HttpException (0x80004005): Request is not available in this context]
   System.Web.HttpContext.get_Request() +8790880
   motivationTool.Global.Application_Start(Object sender, EventArgs e) +43

[HttpException (0x80004005): Request is not available in this context]
   System.Web.HttpApplicationFactory.EnsureAppStartCalledForIntegratedMode(HttpContext context, HttpApplication app) +2722546
   System.Web.HttpApplication.RegisterEventSubscriptionsWithIIS(IntPtr appContext, HttpContext context, MethodInfo[] handlers) +128
   System.Web.HttpApplication.InitSpecial(HttpApplicationState state, MethodInfo[] handlers, IntPtr appContext, HttpContext context) +188
   System.Web.HttpApplicationFactory.GetSpecialApplicationInstance(IntPtr appContext, HttpContext context) +295
   System.Web.HttpApplicationFactory.GetPipelineApplicationInstance(IntPtr appContext, HttpContext context) +56
   System.Web.Hosting.PipelineRuntime.InitializeApplication(IntPtr appContext) +231

[HttpException (0x80004005): Request is not available in this context]
   System.Web.HttpRuntime.FirstRequestInit(HttpContext context) +8894031
   System.Web.HttpRuntime.EnsureFirstRequestInit(HttpContext context) +85
   System.Web.HttpRuntime.ProcessRequestNotificationPrivate(IIS7WorkerRequest wr, HttpContext context) +333

 


--------------------------------------------------------------------------------
Version Information: Microsoft .NET Framework Version:2.0.50727.4200; ASP.NET Version:2.0.50727.4016 

 

Text of virtual directory Web.config

Code:

<?xml version="1.0" encoding="utf-8" ?>
<configuration>
  <system.web>
    <compilation defaultLanguage="c#" debug="true" />
    <customErrors mode="Off" /> 
    <authentication mode="Windows" /> 
    <authorization>
        <allow users="*" />
    </authorization>
    <trace enabled="false" requestLimit="10" pageOutput="false" traceMode="SortByTime" localOnly="true" />
    <sessionState  mode="InProc" stateConnectionString="tcpip=127.0.0.1:42424" sqlConnectionString="data source=127.0.0.1;Trusted_Connection=yes" cookieless="false" timeout="20" />
    <globalization requestEncoding="utf-8" responseEncoding="utf-8" />
 </system.web>
</configuration>